Arocell Products

XPA-210 - a new, universal, cytosolic cell proliferation marker for immunohistochemistry

Background

XPA-210 is a patented peptide marker derived from the well-known, cell proliferation enzyme Thymidine kinase 1 (TK1). The enzyme is part of the pyrimidine salvage pathway, active in DNA synthesis and repair. TK1 is expressed in proliferating cells during S-phase and rapidly degraded during M-phase. The designed antibodies anti-XPA-210-A provide an attractive opportunity for cancer- and tumor- research, as they have been shown to be useful in both serological and immuno-histochemical detection of cytosolic TK1 and cell proliferation in many types of cancer diseases.

Conclusion

Antibodies anti-XPA-210-A were used in immunohistological staining of human tissue slides for specific staining of proliferating cells. The antibodies were used both in a manual protocol and in an automated protocol, based on the DakoCytomation Autostainer system. The specifi city of anti-XPA-210-A was demonstrated in a competition experiment, where staining was performed in the presence or absence of the peptide antigen to which the XPA-210-antibodies were raised. Staining of proliferating cells was show in slides of tonsil, prostate, breast cancer, testis showing broad applicability of the new, universal cell proliferation marker XPA-210. In comparative experiments, XPA-210 (cytosolic) was compared to Ki-67 (nuclear). These experiments show that XPA-210 is a complementary marker to the well-established Ki-67 by providing staining of the cytoplasm in proliferating cells.